dapi containing antifade mounting solution prolong gold  (Thermo Fisher)

Journal: Pharmaceutics

Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

doi: 10.3390/pharmaceutics15061651

Figure Lengend Snippet: Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) Representative fluorescence microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). DAPI-stained nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.

Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

Techniques: Incubation, Fluorescence, Microscopy, Transmission Assay, Staining

Journal: Pharmaceutics

Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

doi: 10.3390/pharmaceutics15061651

Figure Lengend Snippet: Digitoxin-loaded hTf-QDs reduced A549 cell number. Left panel: Representative fluorescence microscopy images (20×) of 16HB14o- and A549 cells incubated for 48 h without (Control, ( A , B ) panels) or with hTf-QDs (( C , D ) panels). hTf-QDs are visualized in red and DAPI-stained nuclei are shown in blue. ( E ) Effect of hTf-QDs loaded with digitoxin (hTf-QDs-Dig) on 16HB14o- (green) and A549 (red) cells treated for 48 h. As a control, cells were incubated without hTf-QDs (Control) or in the presence of hTf-QDs without digitoxin (hTf-QDs). * indicates significative difference between treatments.

Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

Techniques: Fluorescence, Microscopy, Incubation, Staining